Detalles del Título
Detalles del Título

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Título Biotechnology: DNA to protein: a laboratory project in molecular biologyLibros / Impreso - Libros
Autor(es) Thiel, Teresa (Autor)
Bissen, Shirley (Autor)
Lyons, Eilene M. (Autor)
Publicación New York, NY : McGraw-Hill Higher Education, ©2002
Descripción Física x, 182 páginas : ilustraciones ; 28 cm.
Idioma Inglés;
ISBN 0072416645
Clasificación(es) 572.8
BIOLOGIA
Materia(s) Biología molecular; Clonación; Clonación molecular; Manuales de laboratorio; Genética molecular; Amilasas;
Nota(s) Incluye referencias bibliográficas e índice
CONTENIDO: Examine Enzyme Activity of alpha-Amylase -- Starch Plate Assay -- Quantitative Enzyme Assay -- Factors Affecting Enzyme Function -- Examine alpha-Amylase Proteins -- Analysis of Protein Structure Using RasMol -- Analysis of alpha-Amylase Proteins -- SDS-PAGE -- Western Blotting -- Examine DNA Structure -- Analysis of DNA Structure Using RasMol -- Isolation of Chromosomal DNA from Bacillus licheniformis -- Find the alpha-Amylase Gene -- PCR Amplification and Labeling of Probe DNA -- Southern Hybridization -- Restriction Enzyme Cleavage of Chromosomal DNA -- Denaturation and Transfer of DNA to a Membrane -- Southern Hybridization and Detection -- Clone the alpha-Amylase Gene -- Cloning the alpha-Amylase Gene -- Cleavage of Chromosomal DNA -- Cleavage of Plasmid DNA -- Ligation of Chromosomal and Plasmid DNA - Transformation -- Identification of alpha-Amylase Clones -- Analyze alpha-Amylase Clones -- Verification and Mapping of alpha-Amylase Clones -- Verification of alpha-Amylase Clones Using PCR -- Isolation of Plasmid DNA from alpha-Amylase Clones -- Restriction Cleavage and Mapping of alpha-Amylase Plasmid DNA -- Preservation of Recombinant Strains -- Southern Analysis of alpha-Amylase Plasmid DNA -- Enzyme Activity of alpha-Amylase Clones
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Disponibilidad
CodBarras Localización Piso Signatura Estado Categoría
093324Biblioteca Universidad Icesi2572.8/T431DisponibleCol. General