Detalles del Título
Detalles del Título

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Título Basic methods in protein purification and analysis: a laboratory manualLibros / Impreso - Libros
Autor(es) Simpson, Richard J. (Editor)
Adams, Peter D. (Editor)
Golemis, Erica A. (Editor)
Publicación Cold Spring Harbor, New York : Cold Spring Harbor Laboratory Press, ©2009
Descripción Física vii, 436 páginas : ilustraciones ; 28 cm.
Idioma Inglés;
ISBN 0879698675
Clasificación(es) 572.6078
QUIMICA
Materia(s) Proteínas - Purificación - Manuales de laboratorio; Proteínas - Análisis - Manuales de laboratorio;
Nota(s) Incluye referencias bibliográficas e índice.
CONTENIDO: Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- Purification using gel electrophoresis -- One-dimensional polycrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Purification using column chromatography -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Characterizing protein complexes -- Identification of protein-protein interactions by conventional column chromatography -- identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-S transferance fusion proteins -- Direct visualization of protein intractions in living cells using bimolecular fluorescence complementation analysis -- In silico tools: analysis for creating focused interaction networks.
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110184Biblioteca Universidad Icesi2572.6078/B311bDisponibleCol. General